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Yourlocation: Home > News > Enzymatic Hydrolysates of Corn Stover Pretreated by the Ionic Liquids/N-Methyl pyrrolidone Solution for Microbial Lipid Production
Enzymatic Hydrolysates of Corn Stover Pretreated by the Ionic Liquids/N-Methyl pyrrolidone Solution for Microbial Lipid Production.Efficient transformation of lignocellulosic materials into sugars has been intensively pursued for the production of biofuels and chemicals by microbial conversion.[1] In this study, we show that corn stover can be effectively dissolved in the solution of ionic liquids in N-Methyl pyrrolidone  (NMP), and that the enzymatic hydrolysates of the regenerated materials are suitable for microbial lipid production by the oleaginous yeast Rhodosporidium Toruloides Y4 (R. toruloides Y4). Up to 10 wt% corn stover was dissolved in EmimAc/N-Methyl pyrrolidone NMP (XILs=0.2, mole fraction of ionic liquids in the mixed solution) at 140 C after 60 min. Physiochemical analysis showed major differences in terms of cellulose crystallinity, compositional distribution and surface morphology between raw corn stover samples and the regenerated materials, indicating that dissolution in the EmimAc/N-Methyl pyrrolidone NMP system followed by regeneration with anti-solvents could be used as an effective pretreatment technology. Enzymatic saccharification of the regenerated corn stover afforded an 82.9% total reducing sugars yield and a 60.8% glucose yield within 24 h. The hydrolysates were used, without detoxification, as the carbon sources for the cultivation of R. toruloides Y4 for lipid production. Glucose and xylose in the hydrolysates were both consumed in our process. The overall lipid yield was estimated as 0.071 g lipid per g raw corn stover, but it is expected to be greatly improved in the future. Our study provides an integrated process to convert lignocellulosic biomass into lipid and should be an appealing route for further optimization and development.

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